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. 2003 Sep;47(9):2997–3001. doi: 10.1128/AAC.47.9.2997-3001.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 3. — Killing curve of tcp mutants in the presence of biapenem at 8 μg/ml. Assuming that the survival at zero time was 100% (1 × 10⁹ to 2 × 10⁹ CFU/ml), the number of CFU was changed to a percentage. (A) Killing curve of tcp knockout mutant. [], P. aeruginosa SM7 (parent strain for KMX7803); [], P. aeruginosa KMX7803 (tcp::Tn1737KH); [], P. aeruginosa SM21 (parent strain for TKP011); [▪ ▪ ▪ ▪□▪ ▪ ▪ ▪], P. aeruginosa TKP011 (tcp::Gm^r) (B) Complementation of tcp gene. [], P. aeruginosa SM21; [▪ ▪ ▪ ▪□▪ ▪ ▪ ▪], P. aeruginosa TKP011 (tcp::Gm^r); [▪ ▪ ▪ ▪⧫▪ ▪ ▪ ▪], P. aeruginosa TKP04 (tcp::Gm^r, pTKM04).

Inline graphic — Killing curve of tcp mutants in the presence of biapenem at 8 μg/ml. Assuming that the survival at zero time was 100% (1 × 10⁹ to 2 × 10⁹ CFU/ml), the number of CFU was changed to a percentage. (A) Killing curve of tcp knockout mutant. [], P. aeruginosa SM7 (parent strain for KMX7803); [], P. aeruginosa KMX7803 (tcp::Tn1737KH); [], P. aeruginosa SM21 (parent strain for TKP011); [▪ ▪ ▪ ▪□▪ ▪ ▪ ▪], P. aeruginosa TKP011 (tcp::Gm^r) (B) Complementation of tcp gene. [], P. aeruginosa SM21; [▪ ▪ ▪ ▪□▪ ▪ ▪ ▪], P. aeruginosa TKP011 (tcp::Gm^r); [▪ ▪ ▪ ▪⧫▪ ▪ ▪ ▪], P. aeruginosa TKP04 (tcp::Gm^r, pTKM04).