TABLE 2.
pI values and discriminant amino acids for OXY-1 and OXY-2 β-lactamase groups and the β-lactamases of K. oxytoca strains SG266 and SG271
| β-Lactamase group or strain (n) | pI value(s) | Amino acida at position (Ambler numbering):
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 4 (−) | 9 (−) | 11 (−) | 12 (8) | 15 (11) | 17 (13) | 22 (18) | 25 (21) | 29 (25) | 30 (26) | 31 (27) | 32 (28) | 33 (29) | ||
| OXY-1 group (8)b | 7.1, 7.5, 8.2, 8.8 | S | T | L | M | A | V | A | S | S | A/V | D | A | I |
| OXY-2 group (9)c | 5.2, 5.7, 6.4, 6.5, 6.8 | S | I | M | L | del | V | A | A | S | T | D | A | I |
| SG266 | 7.7 | S | S | L | M | del | V | A | S | S | A | D | T | L |
| SG271 | 6.7 | T | S | L | I | del | L | C | S | N | A | del | del | I |
| Amino acida at position (Ambler numbering):
| ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 38 (34) | 43 (39) | 44 (40) | 54 (50) | 56 (52) | 75 (72) | 90 (−) | 101 (98) | 103 (100) | 111 (108) | 129 (128) | 140 (137) | 143 (140) | 161 (158) | 168 (165) | 171 (168) | 134 (181) | 194 (191) | 230 (227) | 270 (269) | 278 (277) |
| A | R | S | N | A | G | N | K | S | I | A | K | S | V | T | A | T | R | A | Q | E/K |
| T | R | S | N | A | S | N | N | A | I | T | L | G | A | T | T | S | R | E | Q | E |
| A | R | S | N | A | G | H | K | S | I | A | K | G | V | M | A | T | R | A | Q | E |
| A | S | T | D | T | G | N | K | S | V | A | K | G | V | T | A | T | H | A | L | D |
a
Amino acid numbering is based on the sequence of the OXY-1 β-lactamases.
b
Strains SL781 (10), KH66 (25), SG344 (14), 756 (10), SG49 (14), 563 (10), 1879-77 (10), and SG337 (14).
c
Strains SC 10,436 (12), KoKER (22), 11V (10), D488 (10), SL911 (10), ATCC 13182 (14), SG4 (14), SG77 (14), and SG176 (14). Amino acids in boldface are specific for genetic groups or strains.