TABLE 6.

The genes and discriminant function required for classification of isoniazid (INH), thiolactomycin (TLM), triclosan (TRC), or control groups

ORF	Gene name	Description	Avg squared canonical correlationa	INH [D2INH (x)]b	TLM [D2TLM (x)]	TRC [D2TRC (x)]	Control [D2Control (x)]
Constant				−14,987,826	−167,097,220	−79,450,033	−5,513,637
Rv1686c		Probable conserved integral membrane protein ABC transporter	0.3325	−19,099,766	−100,023,236	−36,942,559	−19,032,909
Rv2244	acpM	Meromycolate extension acyl carrier protein	0.6111	110,774,262	408,156,465	243,639,840	72,779,372
Rv0677c	mmpS5	Possible conserved membrane protein	0.8533	121,600,815	512,108,617	316,180,097	94,776,642
Rv2190c		Conserved hypothetical protein	0.8904	−68,508,665	−286,328,959	−181,659,649	−52,963,842
Rv3250c	rubB	Probable rubredoxin	0.8954	−10,150,989	−44,990,430	−22,707,705	−8,353,423
Rv2276	cyp121	Cytochrome P450 121	0.9040	−36,833,310	−220,327,670	−143,773,133	−43,835,221
Rv3049c		Probable monooxygenase	0.9491	−114,909,397	−452,847,559	−265,224,702	−82,194,446
Rv0208c		Hypothetical methyltransferase	0.9762	−178,128,313	−821,139,430	−524,976,195	−155,439,247
Rv2745c		Possible transcriptional regulatory protein	0.9817	−77,131,242	−323,842,646	−200,033,735	−59,890,920
Rv2253		Possible secreted unknown protein	0.9834	−49,162,506	−201,531,571	−124,495,122	−37,049,967
Rv1072		Probable conserved transmembrane protein	0.9843	64,061,474	246,735,357	152,824,150	44,629,671
Rv0298		Hypothetical protein	0.9865	47,211,509	172,163,109	103,076,691	30,611,076
Rv3341	metA	Probable homoserine O-acetyltransferase	0.9867	1,400,025	−26,153,983	−29,413,890	−6,482,707
Rv0001	dnaA	Chromosomal replication initiator protein	0.9932	−23,069,217	−86,660,290	−56,244,876	−15,604,890
Rv3457c	rpoA	Probable DNA-directed RNA polymerase (α chain)	0.9942	−82,964,894	−232,795,471	−118,734,295	−37,450,414
Rv1737c	narK2	Possible nitrate/nitrite transporter	0.9946	−22,730,982	−62,077,538	−26,268,313	−9,791,891
Rv1871c		Conserved hypothetical protein	0.9967	16,814,613	46,910,792	21,021,772	7,485,856
Rv3404c		Conserved hypothetical protein	0.9971	46,788,129	124,430,283	61,274,979	19,520,565
Rv3486		Conserved hypothetical protein	0.9972	−63,601,803	−175,065,603	−83,869,339	−27,853,590
Rv3310		Possible acid phosphatase	0.9998	35,921,002	97,302,396	47,325,500	15,382,806
Rv0283		Possible conserved membrane protein	1.0000	22,910,896	62,309,712	30,137,464	9,865,584

^aCumulative canonical correlation showing the increasing amount of variance accounted for by each gene in the discriminant model.

^bTo classify an observation (x) into one of the groups, the discriminant score [D²_y (x)] is calculated for each group (y) using the above functions. These scores are inversely related to the distance that observation is from the middle of each group. The observation belongs to the group whose function gives the highest discriminant score. The probability of an observation (x) belonging to a group (y) is calculated as e^D_y2(x)/[e^{D_Control2(x)}+e^D_INH2(x)+e^D_TLM2(x)+e^D_TRC2(x)].