TABLE 1.
Starting vol (ml) | Vol (ml) of filtrate collected | Dextran vol (ml) added to formulation | Final Vol (ml) | Expected SbV concn (mg/ml) after dilution with dextran |
---|---|---|---|---|
700 | 450 | 250 | 500 | 13.5 |
500 | 200 | 100 | 400 | 10.125 |
400 | 150 | 100 | 350 | 7.232 |
350 | 100 | 150 | 400 | 4.52 |
400 | 50 | 350 | 400 | 2.26 |
A total of 700 ml of SSG-NIV (27 mg of SbV/ml) was processed to produce SSG-NIV-dextran. The expected SbV concentration at each step is shown.