FIG. 3.

Gel autoradiogram showing the protection effect at nucleotide U2506 in E. coli 23S rRNA caused by tiamulin binding to CN2476 (wild-type [wt]) and JB5 (L3 mutant) ribosomes. Dideoxy sequencing lanes are indicated by G, A, U, and C. Lanes are labeled to denote reactions with chemically unmodified 70S ribosomes in the absence of tiamulin (R_u), 70S ribosomes modified with CMCT in the absence of tiamulin (R_m), or 70S ribosomes modified in the presence of tiamulin (T_0.2, T_0.5, T₂ or T₁₀, where the subscript indicates the drug concentration [micromolar]). The arrow shows the altered reactivity at position U2506.