TABLE 1.
E. coli strains and plasmids
| Strain or plasmid | Relevant genotype and characteristics | Reference or source |
|---|---|---|
| Strains | ||
| TA542 | Δ7 (ΔEBHGcADcCc recA56/pTRNA66, pHK-rrnC+) | 1 |
| CN2476 | [CN1709] ade::cam CN1709: F−ara Δ(codB-lac)3 thi | 16, 17 |
| JB5 | [CN2476] rplC | This study |
| CAG5051-55a | nadA57::Tn10 btuB3191::Tn10 zbe-280::Tn10 trpB83::Tn10 zed-3069::Tn10 | 19 |
| CAG8209a | zgh-3075::Tn10 | 19 |
| CAG8160a | thi-39::Tn10 | 19 |
| CAG12152a | zgi-3075::Tn10 | 13 |
| CAG12072a | zha-203::Tn10 | 13 |
| CAG12075a | zhe-3083::Tn10 | 13 |
| PG201a | kefB20::Tn10 | I. Booth via EGSCb |
| Plasmids | ||
| pFK1 | rrnB operon inserted into Tetr gene of pBR322; Ampr | 4 |
| pCN102A | lac promoter and polylinker inserted downstream of the fourth codon of the lacZ gene; Ampr | 15 |
a
Only the position of Tn10 is given.
b
EGSC, E. coli Genetic Stock Center, Yale University, New Haven, Conn.