FIG. 2.
Nucleotide sequence of a 9,590-bp DNA fragment of the natural pILT-3 plasmid of K. pneumoniae ILT-3 containing ISEcp1B, β-lactamase CTX-M-19, and IS903D coding sequences. The deduced amino acid sequence is indicated in single-letter code below the nucleotide sequence. The start codons of the ORFs are indicated by horizontal arrows, and stop codons are indicated by asterisks. The −35 and −10 promoter sequences of the Pc, Pint, and Pa promoters are underlined, as well as the +1 position of the transcriptional start of the blaCTX-M-19 site. The 5-bp duplicated target sites of the putative insertion site for the DNA fragment resulting from an ISEcp1B-mediated transposition process are doubly underlined. RBS indicates the putative ribosome binding site for the cmlA1-like gene, and the 9-amino-acid leader peptide sequence of the variant of cmlA1 cassette is indicated. Core and inverse core sites located at each cassette boundary are boxed. TnpA, transposase gene; iron, gene encoding a putative outer membrane lipoprotein for iron uptake; and Orf1, the gene encoding the putative methyl-accepting chemotaxis protein of Tn1721.