TABLE 2.

Sequences of primers used in this study

Primer	Sequence (5′→3′)	Location	No. as shown in Fig. 1
ISEcp1A	GCAGGTCTTTTTCTGCTCC	ISEcp1B transposase	1
ISEcp1B	TTTCCGCAGCACCGTTTGC	ISEcp1B transposase, reverse primer	2
PRECTX-M-3B	CCGTTTCCGCTATTACAAAC	3′ end of blaCTX-M, reverse primer	3
PROM+	TGCTCTGTGGATAACTTGC	Right part of ISEcp1B including −35 and −10 promoter sequences	4
PROM−	GCAGTCTAAATTCTTCGTG	Right part of ISEcp1B lacking −35 and −10 promoter sequences	5
CTX-M-REV	CCGCGAACATCATCCGTTGC	5′ end of blaCTX-M-19, reverse primer for primer extension experiments	6
IS903-A	CATATGAAATCATCTGCGC	IS903D transposase	7
IS903-B	CCGTAGCGGGTTGTGTTTTC	IS903D transposase, reverse primer	8
INT2F	TCTCGGGTAACATCAAGGCCC	3′ end of the int11 integrase gene	9
3′-CS	AAGCAGACTTGACCTGA	5′ end of the qacEΔ1 gene, reverse primer	10
CTX-MA	ACCGCGATATCGTTGGT	blaCTX-M-19 gene	11
CTX-MB	CGCTTTGCGATGTGCAG	blaCTX-M-19 gene, reverse primer	12
ORF1B	ATACTCTTGCTCATATGGGG	5′ end of ORF1 of Tn1721, reverse primer	13