Figure 3.

RNAi phenotypes. Percentages of F₁ animals with a Nob or Vab phenotype are shown for wild-type (a) or nob-1(ct230) (b) hermaphrodites after injection with double-stranded (ds)RNA sequences from nob-1, php-3, or both. In similar experiments with a him-8 (high-incidence-of-males) strain in place of N2, php-3(RNAi) alone also had no apparent effect on male tail development. Progeny (n = ≈500) were scored 24–96 h after each injection. Negative controls (“none”) are uninjected animals. It is very unlikely that php-3(RNAi) could be crossinterfering with nob-1 function or vice versa, because this phenomenon has been observed only when there is >80% identity in overall nucleotide sequence (30).