FIG. 3.
Induction of HSP70 expression by GGA treatment in vitro. Cells of the A549 human alveolar epithelial cell line were treated with GGA at serial concentrations and times in MEM-1% FCS. (A) HSP70 transcripts formed in a dose- and time-dependent manner. (B) Kinetics of HSP70 synthesis and accumulation under optimal conditions. A549 cells were treated with GGA at 10−5 M for 60 min in MEM-1% FCS and were maintained for the indicated times in MEM-5% FCS. Concurrently, the cells were labeled with [35S]methionine as described in Material and Methods. Samples containing equal amounts of radioactivity were subjected to SDS-PAGE (left panel) and immunoblot analysis with anti-HSP70 antibody (right panel).