FIG. 4.
Effect of GGA on viral protein synthesis in vitro. (A) Kinetics of strain PR8-associated proteins and HSP70 synthesis in PR8-infected A549 cells with GGA treatment (10−5 M for 60 min) or without GGA treatment. After the 1-h PR8 adsorption period, the cells were maintained for the indicated times. Cells labeled with [35S]methionine were lysed and subjected to SDS-PAGE. The asterisk and the arrows indicate HSP70 and NP, respectively. (B) Accumulated levels of HSP70 and viral NP at 12 h p.i. with PR8. Samples obtained at 12 h in the above-described experiment were subjected to immunoblot analysis with anti-HSP70 and anti-NP antibodies. Mock, mock infected.