TABLE 1.
Oligonucleotides for identification of resistance genes
| Primera | Sequence (5′ to 3′)b | Tmc | Length of product (bp) | Reference sequence |
|---|---|---|---|---|
| CAT-F | TCCCAATGGCATCGTAAAGAAC | 58.4 | 293 | Transposon Tn9 from E. coli (GenBank entry J01841) |
| CAT-R | TCGTGGTATTCACTCCAGAGCG | 62.1 | ||
| DHFR-F2 | TTTGATGTCCAACCTGAGCGGG | 60.6 | 189 | E. coli dhfr1b (dfrA14) gene (GenBank entry Z50804) |
| DHFR-R2 | TGCGAAAGCGAAAAACGGCG | 62.7 | ||
| DHFR7-F | GTGTCGAGGAAAGGAATTTCAAGCTC | 59.6 | 191 | E. coli dhfrVII gene Tn5086 (GenBank entry 43090) |
| DHFR7-R | TCACCTTCAACCTCAACGTGAACAG | 59.1 | ||
| DPS-F | TCAAGGCAGATGGCATTCCC | 59.4 | 156 | suII gene from Pasteurella multicoda plasmid pIGI (GenBank entry U57647.1) |
| DPS-R | CGACGAGTTTGGCAGATGATTTC | 60.6 | ||
| DPS1-F | GGATGGGATTTTTCTTGAGCCCCGC | 66.8 | 308 | Transposon Tn21 from E. coli (GenBank entry AF071413) |
| DPS1-R | ATCTAACCCTCGGTCTCTGGCGTCG | 64.3 | ||
| incH-F | CGAAATCGGTCCAACCCATTG | 58.6 | 110 | repHI1A from R27 (EMBL entry M95772) |
| incH-R | CGACAACTCATCAGAAGCGTCAAC | 57.6 | ||
| TEM-Fc | TTTTCGTGTCGCCCTTATTCC | 57.9 | 798 | General β-lactamase (TEM-1) Neisseria meningitidis plasmid pAB6 (GenBank entry AF126482) |
| TEM-R | CGTTCATCCATAGTTGCCTGACTC | 62.7 | ||
| TET-F | GCACTTGTCTCCTGTTTACTCCCC | 64.4 | 687 | Shigella flexneri transposon Tn10 (GenBank entry AF162223.1) |
| TET-R | CCTTGTGGTTATGTTTTGGTTCCG | 61.0 |
a
F, forward primer; R, reverse primer.
b
The reference sequences were assigned by using Entrez Nucleotide at NCBI (http://www.ncbi.nlm.nih.gov/Entrez/). The “T” in bold is a C in the E. coli TEM-1D β-lactamase.
c
Tm, melting temperature.