FIG. 1.

Expression vectors. (A) Peptide intracellular expression vector pKBU-Tet/GST is based on the Clontech pPROTet.E332-lacZ vector. SalI and NheI sites were introduced on a cassette, and then the gene for GST and a 5′ hinge region were inserted into the HindIII and PstI sites. Subsequently, peptides were cloned into the SalI and NheI sites. (B) Protein intracellular expression vector pKBU-Pro-Lar is based on the Clontech pPROLar.A222 vector. NdeI, BspHI, and SmaI sites were introduced on a cassette cloned into the KpnI and PacI sites. Subsequently, target genes of interest were cloned into the NdeI or BspHI and SmaI sites. RBS, ribosome binding site.