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. Author manuscript; available in PMC: 2008 Jan 30.
Published in final edited form as: Sens Actuators B Chem. 2007 Jan 30;121(1):150–157. doi: 10.1016/j.snb.2006.09.044

Figure 4.

Figure 4

Confocal scanner image of Cy3-labeled C. burnetti binding to immobilized AMPs (right). Control antibody for C. burnetti is shown abbreviated as anti-Cox. PBS served as a negative (−) control and anti-chicken IgY as a positive (+) control. Incorporation of anti-chicken IgY allowed comparison of slides between independent experiments. A. Concentration-dependence curves for Cy3-labeled C. burnetti binding to polymyxin B (○) and bactenecin (●). B. Concentration-dependence curves for Cy3-C. burneti binding to cecropin A (●), cecropin B (▼) and cecropin P (■). Error bars indicate standard deviation (n = 4 slides). Fluorescent intensities were normalized with respect to chicken positive control.