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. Author manuscript; available in PMC: 2008 Feb 9.
Published in final edited form as: Mol Cell. 2007 Feb 9;25(3):385–397. doi: 10.1016/j.molcel.2007.01.024

Fig 7. Cdc48pNpl4p/Ufd1p promotes Spt23p mobilization from microsomes and segregation of Poly-UbSpt23p120-Spt23p90 complexes in vitro.

Fig 7

Microsome-based mobilization and analysis of mobilized products (A), and bead-based segregation (B) studies were performed as described in the above figure legends for Mga2p, except that epitope-tagged Spt23p proteins carrying the identical tags as Mga2p were used for the studies. (C) Proposed model of Rsp5p-Cdc48pNpl4p/Ufd1p-mediated membrane-bound transcription factor mobilization. Sites of ubiquitin conjugation remained to be identified.