Figure 5.

Gtso dsRNA injection in the regenerative postblastemas inhibits photoreceptor and pigment eye cells but does not affect regeneration of cephalic ganglia. Control (A) and injected (B) organisms. Whole-mount in situ hybridization with opsin riboprobes was performed to visualize the early inhibitory effect of Gtso dsRNA in the photoreceptor cell differentiation after 7 days of regeneration. Control (C) and injected (D) organisms. Sagittal cryosections of differentiated head blastemas after 14 days, stained with toluidine blue, show the same type of cephalic ganglia differentiation, encircled in yellow. cg, cephalic ganglia; d, dorsal; e, eye spot; v, ventral. [Bars = 150 μm (A and B) and 100 μm (C and D)].