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. Author manuscript; available in PMC: 2007 Mar 16.
Published in final edited form as: Pharmacol Ther. 2006 Nov 13;113(1):88–120. doi: 10.1016/j.pharmthera.2006.07.004

Fig. 16.

Fig. 16

The effect of acetoacetate (AA) on CYP2E1 gene transcription. Hepatocytes were treated with PI3K inhibitors, LY294002 (LY) (10–20 μM) or wortmannin (Wort) (100–500 nM), or the PKC inhibitor, bisindolylmaleimide (Bis) (10 μM), for 1.5 h before treatment for 24 h with 5 mM AA. Untreated hepatocytes (UT) were cultured in the absence of AA, PI-3K inhibitors, and the PKC inhibitor. CYP2E1 gene transcription was monitored by hnRNA analysis and band density normalized to the 370bp CYP2E1 internal standard. CYP2E1 hnRNA levels are plotted as a percentage of the CYP2E1 hnRNA levels monitored in untreated hepatocytes. Data are means ± SEM of band densities of 2 or 3 preparations of total RNA *Significantly different from UT, ** significantly different from hepatocytes treated with AA only (p < 0.05).

Reprinted from Abdelmegeed MA, Carruthers NJ, Woodcroft KJ, Kim SK and Novak RF, J Pharmacol Exp Ther. 315, 203–213, 2005, with permission.