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. Author manuscript; available in PMC: 2007 Mar 16.
Published in final edited form as: Mol Cell. 2006 Dec 28;24(6):917–929. doi: 10.1016/j.molcel.2006.12.002

Figure 3.

Figure 3

Post-transcriptional spliceosome assembly is not required for efficient splicing. (A) (Left panel) Primer extension analysis of HA-ADH2 reporter constructs. (Upper right panel) HA-ADH2 pre-mRNAs normalized to endogenous RP51B pre-mRNA. (Lower right panel) Percent pre-mRNA values from HA-ADH2 constructs. (B) U1 snRNP IPs to characterize HZ18-ADH2 pre-mRNA association. Pre-mRNA association is normalized to DBP2 pre-mRNA. Values were then set to 1.0 for GAL10, an intronless negative control. All data represent the average of at least two independent experiments and error bars report average deviation.