Fig. 3.

The effects of infection with Ad.α1DN on VEGF-stimulated NO production. HAECs were infected with 25 Pfu/cell of Ad.Null or Ad.α1DN viruses 24 h prior to experimentation. Cells were incubated in KRH buffer in the presence or absence of 10 ng/ml VEGF₁₆₅ and/or 100 nM wortmannin. After 15 min, medium was removed and assayed for l-NAME-sensitive ${\text{NO}}_2^{-}$ content. The data shown represent the means ± SD NO synthesis from nine independent experiments. *p < 0.05 relative to value in Ad.Null-infected cells.