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. 2007 Mar 20;5(4):e92. doi: 10.1371/journal.pbio.0050092

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) XANES absorption spectra. Top, Mn standards: MnCl₂ (Mn(II)), γ-MnOOH (Mn(III)), and MnO₂ (Mn(IV)). Bottom, D. radiodurans. Control: No IR, analyzed frozen (−14 °C). DR-1: +10 kGy (−78 °C), analyzed frozen (−14 °C). DR-2: +10 kGy (−78 °C), analyzed thawed (5 °C).

(B) pH-dependent IR survival of D. radiodurans. Top, cells were grown in TGY (pH 7), irradiated (⁶⁰Co) in TGY (0 °C) at the indicated pH, neutralized, and then recovered on TGY (pH 7). Bottom, survival of non-irradiated D. radiodurans grown in TGY (pH 7), then held in TGY (0 °C) at the indicated pH for 16 h, neutralized, and then plated on TGY (pH 7). IR survival assays as described previously [1].

(C) XRF elemental distribution maps of Mn and Fe in D. radiodurans. The D. radiodurans diplococcus (designation: no. 109) was isolated from the mid-logarithmic growth phase (OD₆₀₀ 0.3). For additional XRF microprobe analyses (P, Cl, Mn, Fe, Co, Ni, and Cr), see Figure S3.

(D) Transparent image overlay of TEM, LM, and XRF measurements, and ppm contour lines displayed in (C).