Table 2.
HPLC analysis of tuber carotenoids (ng/g dry weight)
| Phytofluene | Lutein | β-carotene | Zeaxanthin | Antheraxanthin | Violaxanthin | Neoxanthin | Other Xanth. | Esters | Total | |
| Wild-type | 61.69 ± 5.60 | 426.00 ± 124.44 | 2.25 ± 1.17 | 320.87 ± 111.63 | 1415.67 ± 335.02 | 1598.73 ± 468.72 | 468.76 ± 161.43 | 76.91 ± 22.60 | 517.06 ± 178.15 | 4887.95 ± 1421.16 |
| Gus 2 | 54.30 ± 5.28 | 501.81 ± 126.33 | 3.11 ± 2.15 | 311.42 ± 98.85 | 1317.06 ± 149.48 | 1282.35 ± 403.34 | 488.24 ± 121.55 | 91.14 ± 21,55 | 615.01 ± 98.73 | 4664.44 ± 1022.00 |
| Fold Variation | 0.88 | 1.18 | 1.38 | 0.97 | 0.93 | 0.80 | 1.04 | 1.18 | 1.19 | 0.95 |
| As-h1 | 162.57 ± 2.88 | 1590.36 ± 257.09 | 85.30 ± 2.33 | 168.37 ± 308.48 | 2198.74 ± 983.80 | 3452.99 ± 749.84 | 1509.40 ± 269.05 | 177.44 ± 54.47 | 4918.76 ± 920.25 | 14263.95 ± 2992.78 |
| Fold Variation | 2.63*** | 3.73 | 37.91*** | 0.52* | 1.55 | 2.16* | 3.22*** | 2.31* | 9.51*** | 2.92*** |
| As-h2 | 55.64 ± 0.58 | 2637.17 ± 570.03 | 34.08 ± 11.71 | 147.90 ± 39.91 | 945.83 ± 156.85 | 802.98 ± 87.20 | 832.59 ± 240.51 | 367.85 ± 19.18 | 4587.20 ± 1069.79 | 10411.26 ± 2195.20 |
| Fold Variation | 0.90 | 6.19*** | 15.15** | 0.46* | 0.67 | 0.50 | 1.78 | 4.78*** | 8.87*** | 2.13** |
| As-h3 | 74.70 ± 8.85 | 2982.04 ± 881.18 | 56.34 ± 16.90 | 37.09 ± 5.84 | 1034.68 ± 402.16 | 11422.26 ± 2470.47 | 754.32 ± 228.82 | 329.06 ± 87.63 | 5068.07 ± 1181.94 | 21758.57 ± 5274.94 |
| Fold Variation | 1.21* | 7.00*** | 25.04*** | 0.12*** | 0.73 | 7.14*** | 1.61 | 4.28*** | 9.80*** | 4.45*** |
| As-h4 | 67.64 ± 24.22 | 1808.90 ± 239.24 | 32.65 ± 6.50 | 153.71 ± 39.99 | 1102.94 ± 287.98 | 3377.31 ± 371.15 | 764.34 ± 171.39 | 255.47 ± 17.75 | 1789.33 ± 51.88 | 9352.31 ± 1186.71 |
| Fold Variation | 1.10 | 4.25*** | 14.51** | 0.48** | 0.78 | 2.11 | 1.63 | 3.32*** | 3.46 | 1.91** |
| As-h5 | 63.30 ± 5.84 | 764.54 ± 244.69 | 2.71 ± 7.75 | 218.53 ± 140.68 | 1382.88 ± 203.52 | 2018.71 ± 282.39 | 456.06 ± 55.76 | 126.36 ± 3.23 | 1293.42 ± 564.92 | 6326.51 ± 1502.94 |
| Fold Variation | 1.03 | 1.79 | 1.20 | 0.68 | 0.98 | 1.26 | 0.97 | 1.64 | 2.50 | 1.29 |
| As-h6 | 73.12 ± 19.18 | 575.11 ± 71.52 | 4.31 ± 0.43 | 331.03 ± 28.14 | 1551.15 ± 108.39 | 1780.21 ± 509.03 | 617.62 ± 103.11 | 120.02 ± 35.45 | 924.89 ± 132.96 | 5977.47 ± 989.06 |
| Fold Variation | 1.18 | 1.35 | 1.92 | 1.03 | 1.10 | 1.11 | 1.32 | 1.56 | 1.79 | 1.22 |
Carotenoids were measured via diode array HPLC (see Methods) on a minimum of 4 different tubers from 2 different plants. Fold variation with respect to the wild-type is reported for each carotenoid compound and for each line. Asterisks indicate significance of the fold variation in an ANOVA test (*: p ≤ 0.05;**: p ≤ 0.01; ***: p ≤ 0.001). For details, see Methods.