Table 4.

Primers used

Primer name	Sequence	Use
AS-chy Up1	TAGAGCTCGGGATTACTTC	AS-chy cloning
AS-chy Dw2	ATGGATCCTCCTTTTCCAA	AS-chy cloning
AS-h Up	GTTAAGGGAACTTCTCCAC	PCR screening
Nos-test 2	CGCGTATTAAATGTATAATTG	PCR screening
AS-h RT Up	ACCCTCCATTTGCCACGAA	Real-time assay
AS-h RT Dw	TTATATGATAATCATCGCAAGACCG	Real-time assay
Chy1 Up	CTTGGCCCAAAACCCACTT	Real-time assay
Chy1 Dw	CCTCAAATTGAGGTTTCAGCTTCT	Real-time assay
Chy2 Up	TTTTGCTGTCTCGAAGAAAGCC	Real-time assay
Chy2 Dw	AGCCAACAGGCAGCTAAACTCT	Real-time assay
Lut5 Up	GTCTCAAGCAAGCAACTTCGTG	Real-time assay
Lut5 Dw	GATAAAAGGTCCATGTGAGCACTG	Real-time assay
Nxs Up	CTTGGAGGAGACTTCTTTGGTGA	Real-time assay
Nxs Dw	CGGAAGTGGTCCTCCCATAG	Real-time assay

Sequences of the primers used for cloning of the gene fragment, for PCR screening of the putative transgenic plants, and for Real Time RT-PCR quantitation of transcript levels. For further details, see Methods.