Figure 4.

Stability of TFPI-2 and asTFPI-2 transcripts. HUVECs and Colo-205 cells were treated with 5 μM actinomycin D for various durations to block transcription. Total RNA was extracted at the indicated time points following the addition of actinomycin D, and quantitative real-time RT-PCR amplification were performed as described in the text to determine copy numbers of each TFPI-2 mRNA. Each data-point represents the average of three amplification reactions performed on a single cDNA sample reverse-transcribed from RNA derived from each sample.