Skip to main content
. Author manuscript; available in PMC: 2007 Mar 19.
Published in final edited form as: Matrix Biol. 2006 Sep 19;26(1):12–19. doi: 10.1016/j.matbio.2006.09.007

Figure 2.

Figure 2

Analyses of lh2 splice variants. A: Alignment of the amino acid sequence encoded by exon 13A of various vertebrate lh2 genes. Hs: Homo sapiens (NP_891988), Mm: Mus musculus (AAK20118), Rn: Rattus norvegicus (XP_579707), Ss: Sus scrofa (ABB02507), Bt: Bos taurus (XP_613110), Gg: Gallus gallus (XP_422695), Dr: Danio rerio. Asterisks indicate residues having 100% identity; closed circles show residues exhibiting conserved changes. B: RT-PCR analysis of zebrafish lh2 splice variant expression at various stages of embryonic development. Top row: amplification of both lh2b (top) and lh2a (bottom) transcripts with primer pair A (see Experimental Procedures). Middle row: unique amplification of lh2b transcripts with primer pair B. Note the decrease in lh2b expression at the 14 somite stage detected with both primer pairs. Bottom row: amplification of actin transcripts (control).