Figure 2.

(A) Typical phosphorimage of a Western blot of whole-cell lysates of T. weissflogii grown under different conditions of pCO₂ and trace metals. (B) Relative TWCA1 levels determined by Western analysis and phosphorimaging in cells grown under different conditions of pCO₂ and Zn′. The graph represents the average of measurements from three independent sets of cultures. All measurements from the same blot were normalized to the value from the 100-ppm, 15-pM Zn culture. (C) Relative amounts of CA activity in lysates of cells grown under different conditions of pCO₂ and trace metal concentrations. The graph represents the average of three measurements from each of two independent sets of cultures. Error bars represent the ranges of values measured (if not shown, error bars are too small to be resolved).