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. 2006 Oct 9;75(1):113–121. doi: 10.1128/IAI.01016-06

FIG. 3.

FIG. 3.

(A) Silver-stained SDS-PAGE of LOS isolated from 35000HP (lanes 1 and 6), 35000HP-RSM2 (lane 2), 35000HP-RSM2(pLBGB) (lane 3), 35000HP-RSM2(pLBGBR) (lane 4), and 35000HP-RSM2(pLBGA) (lane 5). This SDS-PAGE gel shows that only the addition of the lbgA gene changes the LOS glycoforms produced by strain 35000HP-RSM2. (B) Silver-stained SDS-PAGE of LOS isolated from 35000HP-RSM2(pLBGA) (lanes 1 and 2) and 35000HP (lanes 3 and 4). Lanes designated with a “+” are LOS samples that were treated with neuraminidase; lanes designated with a “−” are LOS samples that were not treated with neuraminidase. Neuraminidase treatment of the samples indicates that both the 35000HP LOS and 35000HP-RSM2(pLBGA) LOS have sialylated glycoforms. Various 35000HP LOS glycoforms are labeled in the figures (A5b2, A5b1, A5a1, A5, and A4); see Fig. 1 for details.