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Silver-stained SDS-PAGE of LOS isolated from 35000HP-RSM2 (lane 1), 35000HP-RSM2(pLBGA) (lane 2), 35000HP-RSM223(pLBGA) (lane 3), 35000HP-RSM223 (lane 4), 35000HP-RSM223(pLBGAB) (lane 5), 35000HP-RSM2 (lane 6), and 35000HP (lane 7). This SDS-PAGE gel indicates that the addition of the lbgA gene to the double mutant, 35000HP-RSM223, allows a larger glycoform to be made. Various 35000HP LOS glycoforms are labeled in the figure (A5b2, A5a1, A5, and A4); see Fig. 1 for details.
