TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description or site of isolation | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Host strain used for cloning | Invitrogen |
| DH5αpcnB | pcnB, zad::Tn10 | G. Barcak (30) |
| H. ducreyi | ||
| 35000HP | Human-passaged virulent isolate | 33 |
| 33921 | Kenya | ATCC |
| CIP542 ATCCa | Hanoi, Vietnam | ATCC |
| DMC64 | Bangladesh | 44 |
| DMC111 | Bangladesh | 44 |
| HMC37 | England | 10 |
| HMC65 | Raleigh, NC | 10 |
| HMC112 | Kenya | P. Totten |
| SSMC71 | Bangladesh | 44 |
| 35000HP-RSM2 | lbgB35000HP mutant | 46 |
| 35000HP-RSM223 | lbgB35000HP, lgtA mutant | This study |
| 35000HP-RSM2(pLBGA) | lbgB35000HP mutant + lbgA33921 | This study |
| 35000HP-RSM2(pLBGB) | lbgB35000HP mutant + lbgB33921 | This study |
| 35000HP-RSM2(pLBGBR) | lbgB35000HP mutant + lbgB33921 (insert in the reverse orientation) | This study |
| 35000HP-RSM223(pLBGA) | lbgB, lgtA mutant + lbgA33921 | This study |
| 35000HP-RSM223(pLBGAB) | lbgB, lgtA mutant + lbgAB33921 | This study |
| Plasmids | ||
| pWKS30 | Low-copy-number plasmid vector | 43 |
| pLS88 | H. ducreyi shuttle vector | 9 |
| pCR2.1 | TA cloning vector | Invitrogen |
| pLSSK | H. ducreyi shuttle vector | 45 |
| pUC4DEcat | Source of cat cassette | 17 |
| pRSM2207 | pWKS30 + HincII fragment of 33921 | This study |
| pRSM2287 | pWKS30 + PvuII fragment of 33921 | This study |
| pRSM2279 | lbgB33921 + pCR2.1 | This study |
| pLBGB | lbgB33921 + pLS88 | This study |
| pRSM2306 | lbgA33921 + pCR2.1 | This study |
| pLBGA | lbgA33921 + pLS88 | This study |
| pLBGBR | lbgB33921 + pLS88, insert in the reverse orientation compared to pLBGB | This study |
| pRSM2307 | lbgAB33921 + pCR2.1 | This study |
| pLBGAB | lbgAB33921 + pLSSK | This study |
| pRSM2379 | lgtA + pRSM2072 | 37 |
| pRSM2727 | pRSM2379 with cat gene cloned into the lgtA gene | This study |