FIG. 3.

CCL2 expression in macrophage and DC populations of the spleen after infection by L. monocytogenes. Mice were infected with 5 × 10⁵ CFU of L. monocytogenes. Spleens of mice at 4 and 24 h p.i. or of uninfected controls were removed, macrophage and DC populations were sorted using the indicated markers, and RNA was isolated. RT-PCR or real-time RT-PCR from cDNA was used to study gene expression of CCL2. A. Induction of CCL2 in different macrophage and DC populations as revealed by RT-PCR. B. Quantitation of CCL2 mRNA induction in ERTR-9⁺ and MOMA-1⁺ macrophages using real-time RT-PCR. Cells were sorted from five pooled spleens, and all experiments were repeated at least three times.