FIG. 8.
Colonization of Y. pseudotuberculosis yopH mutants in competition with wild-type Y. pseudotuberculosis 4 days postinoculation. BALB/c mice were inoculated with ∼8 × 102 CFU of an equal mixture of MLF-13 and either the MLF-9, yopH(R409A), yopH(K342A), yopH(V31G), yopH(Q11R), or yopHΔ223-226 strain. Bacteria from lungs were grown on L plates containing X-Gal, and the competitive index was calculated as described in Materials and Methods. Black circles represent the CI from individual mice, open circles indicate that no mutants were detected, and bars represent the geometric mean. P values were determined by a two-tailed Student's t test. *, P < 0.05.