TABLE 2.
LD50 of Y. pseudotuberculosis in intranasal infection of BALB/c mice
| Strain (culture condition) | Dose (no. of CFU/mouse)
|
No. of dead mice/ total no. of mice | Day of death(s) | IgG+a | ||
|---|---|---|---|---|---|---|
| Expt. 1 | Expt. 2 | Expt. 3 | ||||
| IP2666pIB1 (26°C) | 0.8 | 0.8 | 0/7 | 1/4 | ||
| 1.6 | 0/3 | 0/3 | ||||
| 3 | 0/3 | 1/4 | ||||
| 4 | 4 | 2/6 | 15, 17 | 0/3 | ||
| 6 | 0/4 | 0/4 | ||||
| 18 | 2/4 | 16, 18 | 2/2 | |||
| 26 | 26 | 5/6 | 12, 13, 14, 15, 17 | |||
| 61 | 3/3 | 11, 11, 16 | ||||
| 93 | 4/4 | 11, 11, 11, 13 | ||||
| 182 | 3/3 | 11, 12, 13 | ||||
| 460 | 3/3 | 9, 9, 10 | ||||
| 496 | 3/3 | 8, 8,8 | ||||
| 9,280 | 4/4 | 5, 5, 5, 7 | ||||
| IP2666pIB1 (37°C) | 1.3 | 0/3 | ||||
| 1.7 | 0/3 | 0/3 | ||||
| 2.2 | 1/3 | 18 | ||||
| 4.0 | 0/3 | 0/3 | ||||
| 33 | 1/3 | 11 | 2/2 | |||
| 48 | 2/3 | 11, 12 | ||||
| 140 | 3/3 | 9, 9, 10 | ||||
| 2,360 | 3/3 | 5, 5, 6 | ||||
| 3,800 | 3/3 | 5, 5 ,5 | ||||
| IP2666ΔyopH (26°C) | 3 | 0/3 | 0/3 | |||
| 16 | 0/3 | 1/1 | ||||
| 182 | 1/3 | 16 | 2/2 | |||
| 1,192 | 2/3 | 10, 12 | 1/1 | |||
| 9,900 | 3/3 | 9, 10, 10 | ||||
a
No. of seropositive mice/total no. of mice. The number of seropositive mice was determined by enzyme-linked immunosorbent assay against heat-killed Yersinia.