TABLE 2.
Putative M. tuberculosis SigM promoter consensus sequences
| Locus (MT/Rv/annotation)a | Distance from ATG (no. of base pairs) | Putative −35 consensus | Spacer (no. of nucleotides) | Putative −10 consensus | Overexpression level (fold) |
|---|---|---|---|---|---|
| MT0680/Rv0651/rplJ | −315 | GGCGAC | 17 | CGTCGC | 1.4 |
| MT1736/Rv2009 | −294 | TGTAGC | 16 | CGGCGG | 1.7 |
| MT2113/Rv2053c | −95 | TCGATC | 16 | CGTCAT | 1.5 |
| MT2973/Rv2905/lppW | −193 | AGGAAA | 18 | CGTCGA | 1.4 |
| MT3067/Rv2989 | −217 | TCGAAC | 16 | CCTCAT | 1.4 |
| MT3178/Rv3094c* | −40 | GGTAAC | 16 | CGTCAT | 2.7 |
| MT3179/Rv3095 | −330 | CGACAC | 17 | CGTCAC | 1.9 |
| MT3553/Rv3447c* | −89 | CGGAAC | 17 | CGCCCG | 8.4 |
| MT3554/Rv3448* | −298 | AGAAAC | 17 | CGGCGA | 1.7 |
| MT4026/Rv3907c/pcnA* | −95 | CGACAC | 18 | CGGCAA | 1.6 |
| Consensus | GGAAC | CGTCR |
a
Shown is the identification of the consensus promoter sequence for SigM by aligning the promoter regions of the overexpressed genes obtained by microarray. Asterisks represent genes which appear to be arranged in an operon-like fashion, and for these genes only, the sequence upstream of the first gene of the operon was considered for the identification of putative SigM promoter consensus sequences. R indicates A or G.