Table 1.

Time constants and relative amplitudes of membrane potential generation on photoreduction of P. denitrificans COX (wild type and variants)

Reaction	WT, H2O	WT, D2O	D124N	E278Q	K354M
O → E	τ1  = 21.7 ± 5.5 μs	τ1  = 20.6 ± 4.6 μs	τ1  = 19.7 ± 2.9 μs	τ1  = 22.7 ± 6.2 μs	τ1  = 18.0 ± 3.8 μs
	α1  = 0.57 ± 0.10	α1  = 0.67 ± 0.15	α1  = 0.58 ± 0.08	α1  = 0.62 ± 0.11	α1  = 0.79 ± 0.08
	τ2  = 176 ± 50 μs	τ2  = 352 ± 71 μs	τ2  = 163 ± 32 μs	τ2  = 139 ± 29 μs	τ2  = 859 ± 170 μs
	α2  = 0.43 ± 0.10	α2  = 0.33 ± 0.15	α2  = 0.42 ± 0.08	α2  = 0.38 ± 0.11	α2  = 0.21 ± 0.08
O → R	τ1  = 19.6 ± 3.3 μs	τ1  = 21.8 ± 5.1 μs	τ1  = 28.7 ± 6.5 μs	τ1  = 28.8 ± 5.3 μs	τ1  = 31.9 ± 5.6 μs
	τ2  = 154 ± 45 μs	τ2  = 304 ± 75 μs	τ2  = 170 ± 53 μs	τ2  = 168 ± 36 μs	τ2  = 897 ± 181 μs
	τ3  = 1.1 ± 0.3 ms	τ3  = 2.0 ± 0.6 ms	τ3  = 2.1 ± 0.5 ms	τ3  = 1.5 ± 0.6 ms	τ3  = 4.3 ± 0.7 ms
			τ4  = 17.6 ± 5.4 ms	τ4  = 14.9 ± 5.9 ms
(+KCN)	τ1  = 20.9 ± 5.0 μs	τ1  = 20.2 ± 4.7 μs	τ1  = 21.0 ± 4.9 μs	τ1  = 19.6 ± 5.2 μs	τ1  = 18.7 ± 5.4 μs