TABLE 2.
Identification of immunoreactive SCV and LCV proteins
| No. | Gene | Description | Predicted mass (kDa) | Predicted pI | Serum 2036
|
Serum 3004
|
||
|---|---|---|---|---|---|---|---|---|
| Cell variant MS/MS identificationa | Spot in other cell variant lysateb | Cell variant MS/MS identificationa | Spot in other cell variant lysateb | |||||
| 1 | CBU1718 | 60-kDa chaperone (GroEL) | 58.3 | 4.9 | S | + | S+L | NAc |
| 2 | CBU0236 | Elongation factor EF-Tu | 43.5 | 5.2 | NA | S+L | NA | |
| 3 | CBU0229 | Ribosomal protein L7/L12 (RplJ) | 13.2 | 4.4 | S | + | S+L | NA |
| 4 | CBU1943 | ATP synthase F-1 alpha subunit (AtpA) | 58.8 | 6.0 | NA | L | + | |
| 5 | CBU1416 | Repressor protein C2 | 24.3 | 7.6 | L | NA | ||
| 6 | CBU0952 | Acute disease antigen A (AdaA) | 26.0 | 9.3 | L | + | NA | |
| 7 | CBU0963 | Thioredoxin peroxidase (Bcp) | 16.9 | 8.0 | L | NA | ||
| 8 | CBU0737 | Trigger factor (Tig) | 50.2 | 5.1 | +d | L | ||
| 9 | CBU0497 | 3-Oxoacyl-acyl carrier protein synthase II (FabF) | 44.1 | 5.5 | +d | L | + | |
| 10 | CBU1200 | Isocitrate dehydrogenase (Icd) | 46.6 | 6.4 | NA | L | + | |
a
Protein identified by mass spectrometry in lysates of the SCV (S), the LCV (L), or both (S+L).
b
Corresponding spot present in immunoblot of lysates derived from other cell variants is indicated with a +.
c
NA, not applicable.
d
Immunoreactive spot at the same coordinates in both SCV and LCV 2-D immunoblots as those of the protein identified by serum 3004 in the LCV.