FIG. 2.

Western blot analysis of M. catarrhalis isolates. Proteins present in whole-cell preparations were resolved by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and analyzed by Western blotting with PEP1-specific antibodies at a dilution of 1:10,000. Cell lysates were heated at 100°C prior to electrophoresis. The number on the left indicates the molecular mass in kilodaltons. Lanes 1 and 10, O35E; lane 2, O12E; lane 3, TTA24; lane 4, TTA37; lane 5, P44; lane 6, O46E; lane 7, V1171; lane 8, McGHS1; lane 9, FIN2404; lane 11, McGH; lane 12, McGHS2; lane 13, 32P11B1; lane 14, 7P94B1; lane 15, 11P29B1; lane 16, 13P18B1; lane 17, Mc34F.