FIG. 6.

Flow cytometry analysis of recombinant E. coli bacteria. E. coli cells were incubated with normal mouse serum or with murine serum containing antibodies against His.McaP51.650 (51-650 Abs), His.McaP51.333 (51-333 Abs), or His.McaP333.650 (333-650 Abs) at a dilution of 1:25. Bacteria were washed, incubated with FITC-conjugated secondary antibody, and processed as described in Materials and Methods. The x axes represent the level of fluorescence, and the y axes correspond to the particles counted. These recombinant E. coli cells expressed full-length McaP, truncated McaP that lacks aa 53 to 336, or no McaP at all, as indicated at the bottom of the figure.