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. 2006 Nov 6;75(1):314–324. doi: 10.1128/IAI.01330-06

FIG. 7.

FIG. 7.

Inhibition of adherence with anti-McaP antibodies. (A) E. coli cells carrying the plasmid pCC1.3 (gray bar; adherence negative control) or pSVmcaPS62N (all other bars) were preincubated with no antibodies (black bar; adherence positive control) or with normal mouse serum (NMS) as well as murine sera against aa 51 to 333 (α 51-333), aa 333 to 650, and aa 51 to 650 at dilutions of 1:10, 1:25, or 1:250 (open bars). These bacteria were then incubated with monolayers of A549 cells for 3 h prior to washing off unbound bacteria. (B) The WT M. catarrhalis strains O35E, O12E, and TTA37 were incubated with NMS (black bars) or murine sera against aa 51 to 650 (α 51-650 Abs) (open bars) at a dilution of 1:10. These bacteria were then incubated with A549 cells for 5 min. The results are expressed as the means (±standard errors) of the percentages of inoculated bacteria that bound to A549 cells. The asterisks indicate statistically significant decreases in adherence compared to levels for bacteria incubated with NMS.