TABLE 3.
Effect of yfgL deletion on transcription of genes involved in invasion, motility, and intracellular survival of S. enterica serovar Enteritidis
| Strain | Log10 cDNA copy no. of target gene (mean ± SEM)a
|
|||||
|---|---|---|---|---|---|---|
| sipAb | invFb | hilAb | fliDb | sseAc | ssrBc | |
| LA5 | 8.33 ± 0.21 | 7.86 ± 0.08 | 6.89 ± 0.08 | 7.77 ± 0.30 | 6.41 ± 0.06 | 7.79 ± 0.24 |
| LA5yfgL::aphT | 7.12 ± 0.10 | 6.68 ± 0.08 | 5.95 ± 0.21 | 6.33 ± 0.22 | ND | ND |
| LA5yfgL::aphT(pSyfg-eng) | 7.56 ± 0.17 | 7.46 ± 0.13 | 6.26 ± 0.3 | 7.17 ± 0.27 | ND | ND |
| LA5ΔyfgL | 6.53 ± 0.22 | ND | 5.63 ± 0.20 | 6.37 ± 0.08 | 5.10 ± 0.25 | 6.97 ± 0.33 |
| LA5ΔyfgL(pACyfgL) | 8.04 ± 0.14 | ND | 6.89 ± 0.11 | 7.09 ± 0.09 | 6.03 ± 0.17 | 7.56 ± 0.29 |
The mRNA expression of sipA, invF, hilA, fliD, sseA, and ssrB was assessed by real-time RT-PCR. The expression levels of the indicated genes were normalized to the expression level of the housekeeping gene tufA. The results correspond to the means for at least three independent RNA extractions quantified in duplicate. ND, not done.
Expression of the gene was analyzed from bacteria cultured in LB containing 0.3 M NaCl, corresponding to conditions allowing SPI-1 gene expression induction.
Expression of the gene was analyzed from bacteria cultured in LPM, pH 5.8, corresponding to conditions allowing SPI-2 gene expression induction.