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Changes in MAb fluorescence (excitation wavelength, 284 nm; emission wavelength, 341 nm) upon addition of increasing amounts of synthetic (5, 10, or 25 residues) or native γDPGA: ΔF = (F − Fo)/(Fsat − Fo), where Fo is the initial fluorescence intensity, Fsat is the fluorescence intensity at saturation, and F is the fluorescence intensity at the indicated isopeptide concentration.
