TABLE 2.

Immunochemical and protective activities of γDPGA MAbs

MAb	Result for:
	Capsule reaction				Double immunodiffusion with γDPGAa		Fluorescence perturbation		Protective activity
	Outer layer (concn [μg/ml])			Inner layer (75 μg/ml)	High mol wt	Low mol wt	Kd (μM)	Maximum ΔF (%)	ED50 (μg) (95% CIc)	Hill slope (95% CI)
	200	75	25
21Bl	Puffy	Puffy	Negative	None	Strong	Trace	19	24	3,500 (1,100-11,000)	1.1 (−0.01-2.1)
F24F2	Mixed	Rim	Punctate	Yes	Strong	Strong	0.50	39	480 (250-660)	1.3 (0.8-1.8)
F24G7	Rim	Punctate	Punctate	Yes	Reduced	Reduced	0.36	13	1,100 (840-1,500)	1.8 (0.9-2.6)
F26G3	Rim	Rim	Punctate	Yes	Strong	Strong	0.37	23	590 (490-700)	2.1 (1.4-2.8)
F26G4	None	None	None	Yes	Strong	Strong	0.51	32	460 (280-760)	1.7 (0.4-3.0)
F30D1	Rim	Punctate	Punctate	Yes	No pptb	No ppt	0.51	26	1,200 (650-2,300)	0.9 (0.4-1.5)

^aImmunodiffusion was done with γDPGA from B. anthracis.

^bThe absence of a precipitate (ppt) with MAb F30D1 is most likely due to an artifact, i.e., autoaggregation.

^cCI, confidence interval.