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. 2006 Nov 6;75(1):350–357. doi: 10.1128/IAI.01103-06

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Copyright © 2007, American Society for Microbiology

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FIG. 2. — Western blots of whole-cell lysates of S. pneumoniae serotype 2 strain D39 (A) and serotype 6A strain WCH16 (B) showing the reactivities of the antibodies raised against the various protein antigens. The lysates were reacted with specific antisera generated from mice immunized with the various antigens cloned and expressed from capsular type 2 variants of pneumococci. Results for nitrocellulose membranes reacted with sera from mice immunized with alum (lane 1), anti-PdB (lane 2), anti-PspA (lane 3), anti-PspC (lane 4), anti-PhtB (lane 5), and anti-PhtE (lane 6) are shown. The corresponding molecular mass of each of the proteins is indicated: pneumolysin (53 kDa), PspA (86 kDa), PspC (97 kDa), PhtB (100 kDa), and PhtE (115 kDa). See text for full description.