TABLE 1.
Antibody titers (total IgG) obtained from mice immunized with PdB, PspA, PspC, PhtB, and PhtE
| Immunization group | Antibody titera response to indicated protein antigen
|
||||
|---|---|---|---|---|---|
| PdB | PspA | PspC | PhtB | PhtE | |
| Alum | −(<100)b | ||||
| PdB | 5,000 | ||||
| PspA | 2,750 ± 250 | 1,800 ± 200c | |||
| PspC | 3,000 ± 500c | 5,000 ± 1,000 | |||
| PhtB | 10,000 ± 2,000 | 3,000 ± 500c | |||
| PhtE | 2,000 ± 300c | 5,500 ± 1,500 | |||
| PdB+PspA | 5,250 ± 750 | 2,750 ± 250 | ND | ND | ND |
| PdB+PspC | 4,500 ± 500 | ND | 4,500 ± 500 | ND | ND |
| PdB+PhtB | 5,750 ± 1,250 | ND | ND | 8,500 ± 1,500 | ND |
| PspA+PspC | ND | 3,500 ± 500 | 4,200 ± 500 | ND | ND |
| PspA+PhtB | ND | 2,750 ± 250 | ND | 10,000 ± 2,000 | ND |
| PhtB+PhtE | ND | ND | ND | 8,000 ± 1,500 | 7,000 ± 3,000 |
| PdB+PspA+PspC | 5,000 ± 500 | 3,000 ± 500 | 4,000 ± 500 | ND | ND |
| PdB+PspA+PhtB | 4,000 | 2,750 ± 250 | ND | 8,500 ± 1,500 | ND |
a
The antibody titer is the reciprocal of the dilution of serum giving 50% of the highest absorbance value above the background level at 405 nm, as determined by ELISA. ND, not determined.
b
−(<100) denotes no reactivity.
c
Denotes cross-reactive antibodies (see text).