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Macrophage uptake and survival assay. The uptake and survival of ΔwbtDEFFt ▪ compared to SchuS4 ○ (A) or of ΔwbtDEFFn ▴ compared to strain U112 ⋄ (B) in J774 macrophages. Intracellular bacteria were counted as the number of CFU per ml of lysed macrophages at 0, 24, and 48 h postinfection. The assays were completed in triplicate with similar results observed each time.
