TABLE 1.

Oligonucleotide primers for amplification of the E. canis gp19 gene

Recombinant protein	Forward primer	Sequence (5′ to 3′)	Reverse primer	Sequence (5′ to 3′)	Amplicon size (bp)
gp19	P16N-F	CACGTTCAAAATCATGTTGA	P16C-R	CGCACAATCACAACAGTTGT	405
gp19N	P16N-F	CACGTTCAAAATCATGTTGA	P16N-R	GCATACTGGTCTTTCCT	222
gp19N1	P16N-F	CACGTTCAAAATCATGTTGA	p19 132-R	AGATACTTCTTGTAACTCCATT	126
gp19N1-C	Small FOR	CATTTTACTGGTCCTACT	p19 132-R	AGATACTTCTTGTAACTCCATT	72
gp19N2	p19 133-F	TCTATTGATAGTGTAGGATGC	P16N-R	GCATACTGGTCTTTCCT	96
gp19C	P16C-F	GCAGGTTTAGAGAGCTT	P16C-R	CGCACAATCACAACAGTTGT	180