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. 2006 Nov 13;75(2):838–845. doi: 10.1128/IAI.01075-06

FIG. 3.

FIG. 3.

ELISpot detection of IFN-γ-producing splenocytes from BALB/c mice in response to FMP011. BALB/c mice were immunized with 10 μg/mouse of FMP011 formulated in 100 μl AS01B (A) or AS02A (B). The IFN-γ-releasing cells, upon in vitro restimulation with recombinant protein FMP011 (10 μg/ml) or peptide 102 (2 μg/ml), were determined. Results shown are the averages ± standard deviations of eight determinations for the time point 2 weeks after the third immunization (solid bars) and four determinations for the time point 7 weeks after the third immunization (open bars). ANOVA analysis of data obtained show that the responses for splenocytes stimulated with FMP011 or peptide 102 at either 2 or 7 weeks after the third immunization was significantly different from media-alone stimulation (P < 0.05).