FIG. 6.

IFN-γ production in the presence of anti-CD4⁺ or anti-CD8⁺ antibodies. BALB/c mice were immunized with 10 μg FMP011 formulated in 100 μl AS01B. Three weeks after the third immunization, splenocytes were harvested and IFN-γ-producing cells were determined by ELISpot analysis. Before in vitro stimulation with FMP011 blocking antibody against CD4⁺ and/or CD8⁺, markers were added to the culture. Results shown are the averages of four determinations ± standard deviations done in triplicate. There is no significant difference in the response seen in cells treated with anti-CD8 antibodies and control FMP011 cells or between cells treated with anti-CD4 antibodies only and those treated with anti-CD4 and anti-CD8 antibodies. However, the level of IFN-γ production was significantly reduced when cells were treated with any anti-CD4 antibody (P < 0.05).