FIG. 4.

CD4⁺ CD25⁺ T cells are detected in peripheral tissues of naïve and chronically infected mice. Blood and tissue lymphocytes were stained with anti-CD4 and anti-CD25 antibodies, and analyzed by flow cytometry. Mean frequencies of CD4⁺ CD25⁺ T cells as a percentage of CD4⁺ T cells are depicted from (A) blood and spleen of naïve and chronically infected mice (n = 102 naïve blood; n = 23 chronic blood; n = 6, naïve spleen; n = 16, chronic spleen) and (B) tissues of chronically infected mice (n = 16, spleen; n = 14, muscle; n = 10, inguinal lymph nodes; n = 6, popliteal lymph nodes). Error bars represent standard deviations.