FIG. 5.

Analysis of Foxp3 mRNA and scurfin expression. (A) Mice were perfused, total RNA was extracted from spleen, muscle, or lymph node-derived lymphocytes, reverse transcribed, and amplified as described in Materials and Methods. “Sp” and “Mu” refer to FACS-enriched CD45⁺ lymphocytes from the spleen and muscle, respectively, of mice chronically infected with Brazil-strain T. cruzi. “NSp” refers to splenocytes from a naïve mouse, while “CILN” and “CPLN”, respectively, refer to lymphocyte preparations from the inguinal and popliteal lymph nodes of one mouse chronically infected with Brazil-strain T. cruzi. (B) Lymphocytes were isolated from tissues of naïve or chronically infected mice and stained for extracellular CD4 and intracellular scurfin. Mean data from at least 2 mice are depicted here.