FIG. 6.

Treatment of chronically infected mice with anti-CD25 monoclonal antibodies does not increase the frequency of IFN-γ producing CD8⁺ T cells. Mice chronically infected with Brazil strain T. cruzi were given intraperitoneal injections of anti-CD25 MAbs (PC61 clone, n = 2), or mock treated with PBS as a control (n = 2). Mice were perfused and lymphocytes were then isolated from the spleen and muscle tissue. CD45⁺ cells from muscle tissue were FACS-enriched to >97% purity. (A) Analysis of CD4 and CD25 (7D4 clone) expression. (B) Intracellular IFN-γ responses of spleen and muscle-derived CD8⁺ lymphocytes cultured for 15 h in the presence of plate-bound anti-CD3. Figure depicts one of three similar independent experiments.