FIG. 2.
TRYP-specific IgG responses in mice vaccinated with TRYP/TRYP, TRYP/Vec, or Vec/Vec. Mice were vaccinated with two 100-μg doses of TRYP or vector DNA given 3 weeks apart and boosted with 1 × 106 PFU of TRYP or vector MVA 2 weeks later. At 2 weeks after administration of the booster, immune cells were recalled by in vivo inoculation of 5 μg FTP in the hind footpad, and serum, spleens, and draining LN were removed after 48 h. TRYP-specific total IgG (A), IgG1 (B), and IgG2a (C) were analyzed by ELISA using pooled sera. The antibody levels are the averages for duplicate absorbance (OD) determinations obtained using 12 serial twofold dilutions. The dotted line indicates the value used to determine end point titers. The results are representative of the results of replicate experiments in which similar results were obtained.